In vitro Studies of a Multimodal Approach to Cancer Treatment

The body of work presented in this thesis is related to the development and efficacy of new and multimodal treatments for cancer. The relative cytotoxicity as well as selectivity of such modalities is assessed through various cell assay techniques (in- cluding clonogenic assay) by involving separate and co-cultured cell experiments. Two cancer cell lines are used in these studies, the very radioresistant 9L cell line (gliosarcoma brain cancer model) and the Michigan Cancer Foundation-7 (MCF7) cell line (invasive breast ductal carcinoma model). In addition, and where relevant, the Madin-Darby Canine Kidney (MDCK) cell line was also studied as an example of a non-cancerous cell in vitro model for compound, drug and/or nanoparticle ex- posure as well as multimodal treatment response.