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TCTP regulates genotoxic stress and tumorigenicity via intercellular vesicular signaling

journal contribution
posted on 2024-11-17, 12:42 authored by Robert Amson, Andrea Senff-Ribeiro, Teele Karafin, Alexandra Lespagnol, Joane Honoré, Virginie Baylot, Josette Banroques, N Kyle Tanner, Nathalie Chamond, Jordan D Dimitrov, Johan Hoebeke, Nathalie M Droin, Bastien Job, Jonathan Piard, Ulrich Axel Bommer, Kwang Wook Choi, Sara Abdelfatah, Thomas Efferth, Stephanie B Telerman, Felipe Correa Geyer, Jorge Reis-Filho, Adam Telerman
Oncogenic intercellular signaling is regulated by extracellular vesicles (EVs), but the underlying mechanisms remain mostly unclear. Since TCTP (translationally controlled tumor protein) is an EV component, we investigated whether it has a role in genotoxic stress signaling and malignant transformation. By generating a Tctp-inducible knockout mouse model (Tctp–/f–), we report that Tctp is required for genotoxic stress-induced apoptosis signaling via small EVs (sEVs). Human breast cancer cells knocked-down for TCTP show impaired spontaneous EV secretion, thereby reducing sEV-dependent malignant growth. Since Trp53–/– mice are prone to tumor formation, we derived tumor cells from Trp53–/–;Tctp–/f– double mutant mice and describe a drastic decrease in tumori-genicity with concomitant decrease in sEV secretion and content. Remarkably, Trp53–/–;Tctp–/f– mice show highly prolonged survival. Treatment of Trp53–/– mice with sertraline, which inhibits TCTP function, increases their survival. Mechanistically, TCTP binds DDX3, recruiting RNAs, including miRNAs, to sEVs. Our findings establish TCTP as an essential protagonist in the regulation of sEV-signaling in the context of apoptosis and tumorigenicity.

History

Journal title

EMBO Reports

Volume

25

Issue

4

Pagination

1962-1986

Language

English

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