Surface analyses and immune reactivities of major cell wall-associated proteins of Group A Streptococcus

A proteomic analysis was undertaken to identify cell-wall associated proteins of Streptococcus pyogenes. Seventy-four distinct cell-wall associated proteins were identified, 66 of which were novel. Thirty-three proteins were immunoreactive with pooled S. pyogenes-reactive human antisera. Biotinylation of the GAS cell-surface identified 23 cellwall associated proteins that are surface exposed.