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Separation and identification of phosphatidylcholine regioisomers by combining liquid chromatography with a fusion of collision-and ozone-induced dissociation

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posted on 2024-11-16, 06:59 authored by Rachel Kozlowski, Todd MitchellTodd Mitchell, Stephen Blanksby
The differentiation of closely related lipid isomers is increasingly important to our evolving understanding of lipid biochemistry but it is equally challenging to contemporary chromatographic and mass spectral analyses. Recently, we described a novel ion-activation approach based on combining collision- with ozone-induced dissociation (CID/OzID) for the identification of the relative acyl chain substitution positions in glycerophospholipids. Here we demonstrate, for the first time, that CID/OzID can be effectively combined with reversed-phase chromatography to enable the separation and unambiguous identification of regioisomeric pairs of phosphatidylcholines that differ only in the arrangement of acyl chains on the glycerol backbone.

Funding

Lipidomics of vision

Australian Research Council

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New laser and mass spectrometry-based tools for comprehensive structural elucidation of lipids and their biomolecular interactions

Australian Research Council

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Development of ozone-induced dissociation for lipidomics workflows

Australian Research Council

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History

Citation

Kozlowski, R. L., Mitchell, T. W. & Blanksby, S. J. (2015). Separation and identification of phosphatidylcholine regioisomers by combining liquid chromatography with a fusion of collision-and ozone-induced dissociation. European Journal of Mass Spectrometry, 21 (3), 191-200.

Journal title

European Journal of Mass Spectrometry

Volume

21

Issue

3

Pagination

191-200

Language

English

RIS ID

104795

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