University of Wollongong
Browse

Injectable phenytoin loaded polymeric microspheres for the control of temporal lobe epilepsy in rats

Download (1.52 MB)
journal contribution
posted on 2024-11-15, 02:30 authored by Jonathan Jiang, Zhilian YueZhilian Yue, Sebastien Bauquier, Alan Lai, Yu Chen, Karen J McLean, Amy J Halliday, Yi Sui, Simon Moulton, Gordon WallaceGordon Wallace, Mark Cook
Epilepsy is a prevalent neurological disorder with a high frequency of drug resistance. While significant advancements have been made in drug delivery systems to overcome anti-epileptic drug resistance, efficacies of materials in biological systems have been poorly studied. The purpose of the study was to evaluate the anti-epileptic effects of injectable poly(epsiloncaprolactone) (PCL) microspheres for controlled release of an anticonvulsant, phenytoin (PHT), in an animal model of epilepsy. Methods: PHT-PCL and Blank-PCL microspheres formulated using an oil-in-water (O/W) emulsion solvent evaporation method were evaluated for particle size, encapsulation efficiency, surface morphology and in-vitro drug release profile. Microspheres with the most suitable morphology and release characteristics were subsequently injected into the hippocampus of a rat tetanus toxin model of temporal lobe epilepsy. Electrocorticography (ECoG)from the cerebral cortex were recorded for all animals. The number of seizure events, severity of seizures, and seizure duration were then compared between the two treatment groups. Results: We have shown that small injections of drug-loaded microspheres are biologically tolerated and released PHT can control seizures for the expected period of time that is in accord with in-vitro release data. Conclusion: The study demonstrated the feasibility of polymer-based delivery systems in controlling focal seizures.

History

Citation

Jiang, J. L., Yue, Z., Bauquier, S. H., Lai, A., Chen, Y., McLean, K. J., Halliday, A. J., Sui, Y., Moulton, S., Wallace, G. G. & Cook, M. J. (2015). Injectable phenytoin loaded polymeric microspheres for the control of temporal lobe epilepsy in rats. Restorative Neurology and Neuroscience, 33 (6), 823-834.

Journal title

Restorative Neurology and Neuroscience

Volume

33

Issue

6

Pagination

823-834

Language

English

RIS ID

104109

Usage metrics

    Categories

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC