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Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins

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posted on 2024-11-14, 15:32 authored by Sam Abraham, James Chin, Huub J M Brouwers, Bernadette Turner, Ren Zhang, Toni A Chapman
Here we report the development of a whole-cell biosensor to detect and quantify the induction of the SOS response activated by DNA-degrading colicins. This biosensor utilizes the SOS-responsive cda promoter to regulate the expression of green fluorescent protein. The biosensor assay revealed induction of stress for all DNA-degrading reference colicins (E2, E7, and E8).

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Citation

Abraham, S., Chin, J., Brouwers, H. J.M.., Turner, B., Zhang, R. & Chapman, T. A.. (2011). Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins. Applied and Environmental Microbiology, 77 (18), 6691-6693.

Journal title

Applied and Environmental Microbiology

Volume

77

Issue

18

Pagination

6691-6693

Publisher website/DOI

Language

English

RIS ID

38671

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