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Engineering of perfusable double-layered vascular structures using contraction of spheroid-embedded hydrogel and electrochemical cell detachment

journal contribution
posted on 2024-11-16, 05:14 authored by Yuka Shimazu, Binbin Zhang, Zhilian YueZhilian Yue, Gordon WallaceGordon Wallace, Junji Fukuda
Perfusable vasculatures are essential for engineering three-dimensional thick tissues and organs in the fields of tissue engineering and regenerative medicine. Here, we describe an approach for the fabrication of double-layered vascular-like structures (DVSs) composed of a monolayer of human vascular endothelial cells (HUVECs) covered with a dense human smooth muscle cell (SMC) layer. HUVECs were attached to a gold needle via the oligopeptide self-assembled monolayer and grown to form a HUVEC monolayer that was subsequently embedded in a photo-crosslinkable gelatin hydrogel containing SMC spheroids in a culture chamber. During four days of culture, the hydrogel significantly contracted and formed a dense SMC layer around the needle. The binding between the HUVEC layer and the gold needle was cleaved by applying a negative potential to desorb the oligopeptide and the needle was extracted from the chamber, resulting in a perfusable DVS composed of HUVEC and SMC layers. The DVS was cultured under perfusion, and the cells in the DVS showed greater expressions of SMC-specific genes compared to those of spheroids. The DVS possessed a dynamic contraction ability in response to acetylcholine as observed in the in vivo SMC layer. This study proposes a promising approach for the fabrication of perfusable vasculatures for the engineering of fully vascularized tissues and organs.

Funding

ARC Centre of Excellence for Electromaterials Science

Australian Research Council

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Citation

Shimazu, Y., Zhang, B., Yue, Z., Wallace, G. G. & Fukuda, J. (2019). Engineering of perfusable double-layered vascular structures using contraction of spheroid-embedded hydrogel and electrochemical cell detachment. Journal of Bioscience and Bioengineering, 127 (1), 114-120.

Journal title

Journal of Bioscience and Bioengineering

Volume

127

Issue

1

Pagination

114-120

Language

English

RIS ID

129375

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