Development and preliminary Evaluation of a Loop-Mediated Isothermal Amplification Procedure for Sensitive Detection of Cryptosporidium Oocysts in Fecal and Water Samples
posted on 2024-11-15, 05:18authored byPanagiotis Karanis, Oriel Thekisoe, Klytaimnistra Kiouptsi, Jerry OngerthJerry Ongerth, Ikuo Igarashi, Noborou Inoue
A loop-mediated isothermal amplification (LAMP) procedure for the detection of Cryptosporidium in environmental and fecal samples was developed and evaluated. This is the first demonstration of LAMP applied to detection of Cryptosporidium. Due to its specificity and simplicity, the method could become a useful diagnostic tool for epidemiologic studies of Cryptosporidium presence.
History
Citation
Karanis, P., Thekisoe, O., Kiouptsi, K., Ongerth, J., Igarashi, I. Inoue, N. (2007). Development and preliminary Evaluation of a Loop-Mediated Isothermal Amplification Procedure for Sensitive Detection of Cryptosporidium Oocysts in Fecal and Water Samples. Applied and Environmental Microbiology, 73 (17), 5660-5662.