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Chromatin Modifiers SET-25 and SET-32 Are Required for Establishment but Not Long-Term Maintenance of Transgenerational Epigenetic Inheritance

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posted on 2024-11-14, 20:47 authored by Rachel Woodhouse, Gabriele Buchmann, Matthew Hoe, Dylan Harney, Jason Low, Mark Larance, Peter Boag, Alyson Ashe
Some epigenetic modifications are inherited from one generation to the next, providing a potential mechanism for the inheritance of environmentally acquired traits. Transgenerational inheritance of RNAi phenotypes in Caenorhabditis elegans provides an excellent model to study this phenomenon, and although studies have implicated both chromatin modifications and small RNA pathways in heritable silencing, their relative contributions remain unclear. Here, we demonstrate that the putative histone methyltransferases SET-25 and SET-32 are required for establishment of a transgenerational silencing signal but not for long-term maintenance of this signal between subsequent generations, suggesting that transgenerational epigenetic inheritance is a multi-step process with distinct genetic requirements for establishment and maintenance of heritable silencing. Furthermore, small RNA sequencing reveals that the abundance of secondary siRNAs (thought to be the effector molecules of heritable silencing) does not correlate with silencing phenotypes. Together, our results suggest that the current mechanistic models of epigenetic inheritance are incomplete. RNAi induces epigenetic silencing that is sometimes transgenerationally inherited. Woodhouse et al. show that the putative histone methyltransferases SET-25 and SET-32 are required to establish the epigenetic silencing signal. However, they are dispensable for the maintenance of silencing in subsequent generations, suggesting that transgenerational epigenetic inheritance is a multi-step process.

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Citation

Woodhouse, R. M., Buchmann, G., Hoe, M., Harney, D. J., Low, J. K. K., Larance, M., Boag, P. R. & Ashe, A. (2018). Chromatin Modifiers SET-25 and SET-32 Are Required for Establishment but Not Long-Term Maintenance of Transgenerational Epigenetic Inheritance. Cell Reports, 25 (8), 2259-2272.

Journal title

Cell Reports

Volume

25

Issue

8

Pagination

2259-227200000

Language

English

RIS ID

131760

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