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Binaphthyl-1,2,3-triazole peptidomimetics with activity against Clostridium difficile and other pathogenic bacteria

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posted on 2024-11-14, 23:36 authored by Steven Wales, Katherine A Hammer, Amy M King, Andrew TagueAndrew Tague, Dena Lyras, Thomas V Riley, Paul KellerPaul Keller, Stephen PyneStephen Pyne
lostridium difficile (C. difficile) is a problematic Gram positive bacterial pathogen causing moderate to severe gastrointestinal infections. Based on a lead binaphthyl-tripeptide dicationic antimicrobial, novel mono-, di- and tri-peptidomimetic analogues targeting C. difficile were designed and synthesized incorporating one, two or three D-configured cationic amino acid residues, with a common 1,2,3-triazole ester isostere at the C-terminus. Copper- and ruthenium-click chemistry facilitated the generation of a 46 compound library for in vitro bioactivity assays, with structure-activity trends over the largest compound subset revealing a clear advantage to triazole-substitution with a linear or branched hydrophobic group. The most active compounds were dicationic-dipeptides where the triazole was substituted with a 4- or 5-cyclohexylmethyl or 4,5-diphenyl moiety, providing MICs of 4 μg mL−1 against three human isolates of C. difficile. Further biological screening revealed significant antimicrobial activity for several compounds against other common bacterial pathogens, both Gram positive and negative, including S. aureus (MICs ≥2 μg mL−1), S. pneumoniae (MICs ≥1 μg mL−1), E. coli (MICs ≥4 μg mL−1), A. baumannii (MICs ≥4 μg mL−1) and vancomycin-resistant E. faecalis (MICs ≥4 μg mL−1).

History

Citation

Wales, S. M., Hammer, K. A., King, A. M., Tague, A., Lyras, D., Riley, T. V., Keller, P. A. & Pyne, S. G. (2015). Binaphthyl-1,2,3-triazole peptidomimetics with activity against Clostridium difficile and other pathogenic bacteria. Organic and Biomolecular Chemistry, 13 (20), 5743-5756.

Journal title

Organic and Biomolecular Chemistry

Volume

13

Issue

20

Pagination

5743-5756

Language

English

RIS ID

100920

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