An extraction of the violacein sequence from pJP101 for expression and analysis in pHSG398

Violacein is a pigment found in Chromobacterium violaceum, it has a number of properties, such as; anti-bacterial, anti-viral, anti-protozoa, anti-tumoral. Violacein is produced by a series of genes named vioA, vioB, vioC, vioD and vioE. A violacein sequence is 8 kilo-bases (kb) in size. The goal of this study was to isolate the smallest possible fragment of 'Deoxyribonucleic acid' (DNA) that would produce violacein from pJP101 and making a connection to DNA biological logic gates.