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Advances and unresolved challenges in the structural characterization of isomeric lipids

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posted on 2024-11-16, 05:48 authored by Sarah HancockSarah Hancock, Berwyck Poad, Amani M Batarseh, Sarah AbbottSarah Abbott, Todd MitchellTodd Mitchell
As the field of lipidomics grows and its application becomes wide and varied it is important that we don't forget its foundation, i.e. the identification and measurement of molecular lipids.Advances in liquid chromatography and the emergence of ion mobility as a useful tool in lipid analysis are allowing greater separation of lipid isomers than ever before. At the same time, novel ion activation techniques, such as ozone-induced dissociation, are pushing lipid structural characterization by mass spectrometry to new levels. Nevertheless, the quantitative capacity of these techniques is yet to be proven and further refinements are required to unravel the high level of lipid complexity found in biological samples.At present there is no one technique capable of providing full structural characterization of lipids from a biological sample. There are however, numerous techniques now available (as discussed in this review) that could be deployed in a targeted approach. Moving forward, the combination of advanced separation and ion activation techniques is likely to provide mass spectrometry-based lipidomics with its best opportunity to achieve complete molecular-level lipid characterization and measurement from complex mixtures.

Funding

Developing next generation technologies for unmasking the lipidome

Australian Research Council

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Examination of unique tear lipids and their role in the tear film's structure and function

Australian Research Council

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History

Citation

Hancock, S. E., Poad, B. L.J., Batarseh, A., Abbott, S. K. & Mitchell, T. W. (2017). Advances and unresolved challenges in the structural characterization of isomeric lipids. Analytical Biochemistry, 524 45-55.

Journal title

Analytical Biochemistry

Volume

524

Pagination

45-55

Language

English

RIS ID

111807

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