Culturing and Cryobanking Human Neural Stem Cells

Authors

Jeremy Micah Crook, University of WollongongFollow
Eva Tomaskovic-Crook, University of WollongongFollow

RIS ID

113468

Publication Details

Crook, J. M. & Tomaskovic-Crook, E. (2017). Culturing and Cryobanking Human Neural Stem Cells. In J. M. Crook & T. E. Ludwig (Eds.), Stem Cell Banking: Concepts and Protocols (pp. 199-206). New York, United States: Humana Press.

Abstract

The discovery and study of human neural stem cells has advanced our understanding of human neurogenesis, and the development of novel therapeutics based on neural cell replacement. Here, we describe methods to culture and cryopreserve human neural stem cells (hNSCs) for expansion and banking. Importantly, the protocols ensure that the multipotency of hNSCs is preserved to enable differentiation to neurons and supporting neuroglia.

Grant Number

ARC/CE140100012