Target DNA recognition using electrochemical impedance spectroscopy

Authors

Pauline Michaels, University of New South Wales
Simone Ciampi, University of New South WalesFollow
Chan Yean Yean, Universiti Sains Malaysia
J Justin Gooding, University of New South WalesFollow

RIS ID

105074

Publication Details

Michaels, P., Ciampi, S., Yean, C. Yean. & Gooding, J. Justin. (2010). Target DNA recognition using electrochemical impedance spectroscopy. Nanoscience and Nanotechnology (ICONN), 2010 International Conference on (pp. 282-284). United States: Institute of Electrical and Electronics Engineers.

Abstract

Electrochemical impedance spectroscopy was used to differentiate between different target DNA sequences without the use of a redox label or application of large bias voltages. This method relies on the formation of a mixed self-assembled monolayer (SAMs) composed of single-stranded DNA and a diluent, mercaptoethanol. Recognition of the DNA analytes was achieved through the significant increase in conductance for the double stranded DNA, as compared to single-stranded DNA. Non-complementary and single base-pair mismatches were also able to be differentiated. 2010 IEEE.