Year

1997

Degree Name

Doctor of Philosophy

Department

Department of Biology

Abstract

There is a need to produce safe, highly immunogenic vaccines that can elicit both systemic and mucosal immune responses. Control over the type of immune responses elicited following immunization has traditionally been confined to manipulating the immunogenicity of vaccines with adjuvants and altering the immunization route. In doing so, most studies focus predominantly on the ability of vaccines to elicit humoral immune responses. As result, the modulatory effect of immunization route or adjuvant on the immune response, in particular, the nature and extent of changes to leukocyte populations following immunization, and the importance of these changes for eliciting the final immunological outcomes remain poorly characterized. Knowledge of these changes may contribute to the development of safe and effective vaccines. Immunization by the intradermal route elicits strong immune responses in different animal species. Therefore, one aim of this project was to quantify the changes in leukocyte populations in different murine tissues of intradermally immunized mice that may contribute to the adaptive immune responses ultimately elicited. Mice were immunized with either SAMA4, a generic liposome-iscom hybrid skin and mucosal adjuvant (Adjuvant group), outer membrane proteins (OMP) purified from Actinobacillus pleuropneumoniae (OMP Antigen group), SAMA4- adjuvanted OMP (OMP Vaccine group), or PBS (Control group).

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