Stress gene expression in growing pigs following respiratory disease challenge
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This study aimed to better understand the impact of two respiratory pathogens, Mycoplasma hyopneumoniae (MhP) and Pasteurella multocida (Pm), on the stress response as measured by gene expression using qRT-PCR. Sixty-four crossbred female pigs were allocated to individual pens in four rooms. The experiment consisted of four treatments: Control, Pm, Mhp and Mhp followed by Pm. Each treatment group was housed in separate room and procedures were undertaken to minimize the risk of cross-infection between rooms. Inoculation with Mhp occurred on day 0 and Pm on day 21. Blood samples were collected on days -1, 23 and 41 for gene expression. Results showed that respiratory disease upregulated gene expression of SOCS-1, HSP70 and 29a. The combined Mhp+Pm treatment had no additive effect at day 23 for 29a. The expression of some key stress-related genes is indicative of respiratory challenge of at least individual pathogens.