Title

Cell-free synthesis of 15N-labeled proteins for NMR studies

RIS ID

16946

Publication Details

Ozawa, K., Dixon, N. E. & Otting, G. (2005). Cell-free synthesis of 15N-labeled proteins for NMR studies. IUBMB Life, 57 (9), 615-622.

Abstract

Modern cell-free in vitro protein synthesis systems present powerful tools for the synthesis of isotope-labeled proteins in high yields. The production of selectively N-15-labeled proteins from N-15-labeled amino acids is particularly economic and yields are often sufficient to analyze the proteins very quickly by two-dimensional NMR spectra recorded of the crude reaction mixture without concentration or chromatographic purification of the protein. We review methodological aspects of cell-free in vitro protein synthesis based on an Escherichia coli cell extract, in particular with regard to the production of N-15-labeled proteins for analysis by NMR spectroscopy.

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Link to publisher version (DOI)

http://dx.doi.org/10.1080/15216540500217859