Title

Efficient χ-tensor determination and NH assignment of paramagnetic proteins

RIS ID

16840

Publication Details

Schmitz, C., John, M., Park, A., Dixon, N. E., Otting, G., Pintacuda, G. & Huber, T. (2006). Efficient chi-tensor determination and HN assignment of paramagnetic proteins. Journal of Biomolecular N M R, 35 (2), 79-87.

Abstract

Anisotropic magnetic susceptibility tensors χ of paramagnetic metal ions are manifested in pseudocontact shifts, residual dipolar couplings, and other paramagnetic observables that present valuable long-range information for structure determinations of protein-ligand complexes. A program was developed for automatic determination of the χ-tensor anisotropy parameters and amide resonance assignments in proteins labeled with paramagnetic metal ions. The program requires knowledge of the three-dimensional structure of the protein, the backbone resonance assignments of the diamagnetic protein, and a pair of 2D 15N-HSQC or 3D HNCO spectra recorded with and without paramagnetic metal ion. It allows the determination of reliable χ-tensor anisotropy parameters from 2D spectra of uniformly 15N-labeled proteins of fairly high molecular weight. Examples are shown for the 185-residue N-terminal domain of the subunit ε from E. coli DNA polymerase III in complex with the subunit θ and La3+ in its diamagnetic and Dy3+, Tb3+, and Er3+ in its paramagnetic form.

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Link to publisher version (DOI)

http://dx.doi.org/10.1007/s10858-006-9002-4