GC/GC-MS analysis, isolation and identification of bioactive essential oil components from the Bhutanese medicinal plant, Pleurospermum amabile

Authors

Phurpa Wangchuk, University of WollongongFollow
Paul A. Keller, University of WollongongFollow
Stephen G. Pyne, University of WollongongFollow
Malai Taweechotipatr, Srinakharinwirot University
Sumalee Kamchonwongpaisan, BIOTEC, Thailand

RIS ID

84645

Publication Details

Wangchuk, P., Keller, P. A., Pyne, S. G., Taweechotipatr, M. & Kamchonwongpaisan, S. (2013). GC/GC-MS analysis, isolation and identification of bioactive essential oil components from the Bhutanese medicinal plant, Pleurospermum amabile. Natural Product Communications: an international journal for communications and reviews, 8 (9), 1305-1308.

Abstract

We have hydrodistilled the essential oil (EO) from the aerial parts of the Bhutanese medicinal plant, Pleurospermum amabile using a Clevenger apparatus and evaluated this EO by GC/GC-MS and NMR analysis followed by testing for bioactivity. The GC-MS analysis identified 52 compounds with (E)-isomyristicin as a major component (32.2%). Repeated purification yielded four compounds; (E)-isomyristicin (1), (E)-isoapiol (2), methyl eugenol (3) and (E)-isoelemicin (4). Compound 2 and the mother EO showed the best antiplasmodial activity against the Plasmodium falciparum strains, TM4/8.2 (chloroquine and antifolate sensitive) and K1CB1 (multidrug resistant). They exhibited mild antibacterial activity against Bacillus subtilis. None of the test samples showed cytotoxicity.