Characterization of gellan gum by capillary electrophoresis

Authors

Danielle L. Taylor, University Of Western Sydney
Cameron J. Ferris, University of WollongongFollow
Alison R. Maniego, University Of Western Sydney
Patrice Castignolles, University Of Western Sydney
Marc in het Panhuis, University of WollongongFollow
Marianne Gaborieau, University Of Western Sydney

RIS ID

67608

Publication Details

Taylor, D. L., Ferris, C. J., Maniego, A. R., Castignolles, P., in het Panhuis, M. & Gaborieau, M. (2012). Characterization of gellan gum by capillary electrophoresis. Australian Journal of Chemistry: an international journal for chemical science, 65 (8), 1156-1164.

Abstract

Gellan gums were characterised for the first time using free-solution capillary electrophoresis (CE) or CE under critical conditions (CE-CC). CE-CC is a fast method that separates the polysaccharide. Gellan gums are shown to be heterogeneous in terms of their electrophoretic mobility at 55°C revealing: oligomer peak(s), broad peaks of polymers with a random coil conformation with different degrees of acylation (composition), aggregates, and polymers with double-helix conformation. CE-CC is complementary with the rheological analysis also performed in this work. Sonication of gellan gums is shown to decrease the viscosity of gellan gum mainly by breaking up aggregates. The effect of sonication is stronger on the high-acyl gellan gum since the latter has a far higher tendency to aggregate.