Title
Epidermal Langerhans' cell induction of immunity against an ultraviolet-induced skin tumour
Document Type
Journal Article
RIS ID
31129
Abstract
Langerhans' cells (LC) have been shown experimentally to induce immune responses against many antigens; however, their role in the initiation of anti-tumour immunity has received little attention. This study examined the ability of murine epidermal LC to induce immunity to an ultraviolet radiation (UV)-induced skin tumour. Freshly prepared epidermal cells (EC) were cultured for 2 or 20 hr with granulocyte-macrophage colony-stimulating factor (GM-CSF), pulsed with an extract of the UV-13-1 tumour, then used to immunize naive syngeneic mice. Delayed type hypersensitivity (DTH) was elicited 10 days after immunization by injection of UV-13-1 tumour cells into the ear pinna, and measured 24 hr later. EC cultured with GM-CSF for 2 hr induced antitumour DTH, as did EC cultured for 20 hr without GM-CSF. Conversely, EC cultured for 2 hr without GM-CSF, or EC cultured for 20 hr with GM-CSF were unable to induce a DTH. Induction of immunity required active presentation of tumour antigens by Ia(+) EC and was tumour specific. Thus Ia(+) epidermal cells are capable of inducing anti-tumour immunity to W-induced skin tumours, but only when they contact antigen in particular states of maturation.
This record is in the process of being updated. Please contact us for more information.
Publication Details
Cavanagh, L. L., Sluyter, R., Henderson, K. G., Barnetson, R. StC. & Halliday, G. M. (1996). Epidermal Langerhans' cell induction of immunity against an ultraviolet-induced skin tumour. Clinical and Experimental Allergy, 87 (3), 475-480.