Identification of Salvia species by nrDNA ITS and cpDNA rpl16 sequence analyses
RIS ID
27131
Abstract
Three DNA regions were sequenced for testing six fresh plant samples of Salvia species . These three DNA regions were nrDNA ITS ( nuclear ribosomal DNA internal transcribed spacer), chloroplast rpl16 ( the gene encoding ribosomal protein L16 ) , and trnL- trnF ( the cpDNA region comprising the trnL and the intergenic spacer between trnL and trnF) . The results showed that the nrDNA ITS and rpl16 genes could provide novel information for origin identification of Salvia species. Due to their higher mutation rates of these 2 gene markers, Salvia species-specific primers were designed and S. miltiorrhiza and S. yunnanensis were identified. The trnL- trnF gene expressed low mutation rate, it could not identify the species . Since the damage of DNA by the pretreatments of the dry roots of Chinese herbs, it is hard to apply the molecular markers to commercial samples for identification.
Publication Details
Guo, H., Kan, X., Zhang, R. & Chen, H. (2008). Identification of Salvia species by nrDNA ITS and cpDNA rpl16 sequence analyses. Acta Botanica Yunnanica, 30 (3), 345-350.