Basal and insulin-mediated carbohydrate metabolism in human muscle deficient in phosphofructokinase 1
RIS ID
61854
Abstract
Biopsies were obtained from the quadriceps femoris muscle of two male patients deficient in phosphofructokinase (PFK) 1. In the basal state the patients had markedly higher contents of UDP-glucose (~5-fold), hexose monophosphates (~7- to 13-fold), inosine monophosphate (IMP) (~15-fold), and fructose 2,6-bisphosphate (F-2,6-P 2; ~6-fold) than controls. Fructose 1,6-bisphosphate was not detectable, and phosphocreatine was lower (33 and 54 mmol/kg dry wt) than in controls [72 ?? 4 (SD)]. Patients had normal fasting plasma glucose and insulin levels and basal glucose turnover rates and responded normally to a 75-g oral glucose challenge. Patients were also studied during euglycemic hyperinsulinemia (~95 mg/dl; 40 and 400 mU ?? m -2 ?? min -1). Whole body glucose disposal rates were normal during both insulin infusion rates. Biopsies taken after the 400 mU insulin infusion showed decreases in acetylcarnitine and citrate and increases in the fractional activity of glycogen synthase. It is suggested that the high basal levels of F-2,6-P 2 are, at least partly, a consequence of the high levels of fructose 6-phosphate, which will stimulate flux through PFK- and inhibit fructose-2,6-bisphosphatase. The low phosphocreatine and high IMP contents indicate that carbohydrate availability is important for control of high-energy phosphate metabolism, even in the basal state. The insulin-mediated decreases in acetylcarnitine and citrate suggest an activation of the tricarboxylic acid cycle in skeletal muscle but an absence of the normal response to replenish these intermediates.
Publication Details
Katz, A., Spencer, M., Lillioja, S., Yan, Z., Mott, D., Haller, R. & Lewis, S. (1991). Basal and insulin-mediated carbohydrate metabolism in human muscle deficient in phosphofructokinase 1. American Journal of Physiology - Endocrinology and Metabolism, 261 (4 24-4), E473-E478.