Title

Expression and function of neuronal nicotinic ACh receptors in rat microvascular endothelial cells

RIS ID

106018

Publication Details

Mocca, F., Frost, C., Berra-Romani, R., Tanzi, F. & Adams, D. J. (2004). Expression and function of neuronal nicotinic ACh receptors in rat microvascular endothelial cells. American Journal of Physiology: Heart and Circulatory Physiology, 286 (2 55-2), H486-H491.

Abstract

The expression and function of nicotinic ACh receptors (nAChRs) in rat coronary microvascular endothelial cells (CMECs) were examined using RT-PCR and whole cell patch-clamp recording methods. RT-PCR revealed expression of mRNA encoding for the subunits α2, α3, α 4, α5, α7, β2, and β4 but not β3. Focal application of ACh evoked an inward current in isolated CMECs voltage clamped at negative membrane potentials. The current-voltage relationship of the ACh-induced current exhibited marked inward rectification and a reversal potential (E rev) close to 0 mV. The cholinergic agonists nicotine, epibatidine, and cytisine activated membrane currents similar to those evoked by ACh. The nicotine-induced current was abolished by the neuronal nAChR antagonist mecamylamine. The direction and magnitude of the shift in Erev of nicotine- induced current as a function of extracellular Na+ concentration indicate that the nAChR channel is cation selective and follows that predicted by the Goldman-Hodgkin-Katz equation assuming K+/Na+ permeability ratio of 1.11. In fura-2-loaded CMECs, application of ACh, but not of nicotine, elicited a transient increase in intracellular free Ca 2+ concentration. Taken together, these results demonstrate that neuronal nAChR activation by cholinergic agonists evokes an inward current in CMECs carried primarily by Na+, which may contribute to the plasma nicotine-induced changes in microvascular permeability and reactivity induced by elevations in plasma nicotine.

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Link to publisher version (DOI)

http://dx.doi.org/10.1152/ajpheart.00620.2003