Multi-elemental analysis of brain tissue from healthy Wistar rats using sector field inductively coupled plasma mass spectrometry

Authors

Mitchell C. Paul, University of Newcastle
Carl H. Parsons, University of Newcastle
Mike B. Calford, University of Wollongong
Ellak I. von Nagy-Felsobuki, University of Newcastle

RIS ID

80087

Publication Details

Paul, M. C., Parsons, C. H., Calford, M. B. & von Nagy-Felsobuki, E. I. (2004). Multi-elemental analysis of brain tissue from healthy Wistar rats using sector field inductively coupled plasma mass spectrometry. Spectrochimica Acta Part B: Atomic Spectroscopy, 59 (9), 1485-1490.

Abstract

The normal distribution of a range of elements in the brain tissue of healthy Wistar rats was established using sector field inductively coupled plasma mass spectrometry. A protocol was developed to determine concentrations of Ag, Cd, Hg, Pb, Bi, U, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As and Se in specific brain regions. The concentrations of these elements were determined in the range of 2±1 (e.g. Cr in diencephalon) to 7558±450 ng m1⁻¹ (e.g. Fe in olfactory bulb). The detection limits of the sixteen elements ranged between 5 and 300 pg m1⁻¹, with U yielding the lowest and Fe the highest value. The validity of the protocol was assessed by the analysis of SRM 1577B Bovine Liver and brain tissue spike recoveries. A principal component analysis was used to reveal elemental patterns of the brain regions.