The effect of exercise on the skeletal muscle phospholipidome of rats fed a high-fat diet

Authors

Todd W. Mitchell, University of WollongongFollow
N. Turner, University of WollongongFollow
Paul Else, University of WollongongFollow
Anthony J. Hulbert, University of WollongongFollow
John Hawley, RMIT University
Jong Sam Lee, Daegu University, South Korea
Clinton Bruce, Baker IDI Heart & Diabetes Institute
Stephen J. Blanksby, University of WollongongFollow

RIS ID

34253

Publication Details

Mitchell, T. W., Turner, N., Else, P.L, Hulbert, A. J., Hawley, J., Lee, J., Bruce, C., & Blanksby, S. J., (2010). The effect of exercise on the skeletal muscle phospholipidome of rats fed a high-fat diet. International Journal of Molecular Sciences, 11 (10), 3954-3964.

Abstract

The aim of this study was to examine the effect of endurance training on skeletal muscle phospholipid molecular species from high-fat fed rats. Twelve female Sprague- Dawley rats were fed a high-fat diet (78.1% energy). The rats were randomly divided into two groups, a sedentary control group and a trained group (125 min of treadmill running at 8 m/min, 4 days/wk for 4 weeks). Forty-eight hours after their last training bout phospholipids were extracted from the red and white vastus lateralis and analyzed by electrospray-ionization mass spectrometry. Exercise training was associated with significant alterations in the relative abundance of a number of phospholipid molecular species. These changes were more prominent in red vastus lateralis than white vastus lateralis. The largest observed change was an increase of ~30% in the abundance of 1-palmitoyl-2-linoleoyl phosphatidylcholine ions in oxidative fibers. Reductions in the relative abundance of a number of phospholipids containing long-chain n-3 polyunsaturated fatty acids were also observed. These data suggest a possible reduction in phospholipid remodeling in the trained animals. This results in a decrease in the phospholipid n-3 to n-6 ratio that may in turn influence endurance capacity.