Development and preliminary Evaluation of a Loop-Mediated Isothermal Amplification Procedure for Sensitive Detection of Cryptosporidium Oocysts in Fecal and Water Samples

Authors

Panagiotis Karanis, Obihiro University of Agriculture and Veterinary Medicine
Oriel Thekisoe, Obihiro University of Agriculture and Veterinary Medicine
Klytaimnistra Kiouptsi, Obihiro University of Agriculture and Veterinary Medicine
Jerry Ongerth, University of WollongongFollow
Ikuo Igarashi, Obihiro University of Agriculture and Veterinary Medicine
Noborou Inoue, Obihiro University of Agriculture and Veterinary Medicine

RIS ID

21821

Publication Details

Karanis, P., Thekisoe, O., Kiouptsi, K., Ongerth, J., Igarashi, I. Inoue, N. (2007). Development and preliminary Evaluation of a Loop-Mediated Isothermal Amplification Procedure for Sensitive Detection of Cryptosporidium Oocysts in Fecal and Water Samples. Applied and Environmental Microbiology, 73 (17), 5660-5662.

Abstract

A loop-mediated isothermal amplification (LAMP) procedure for the detection of Cryptosporidium in environmental and fecal samples was developed and evaluated. This is the first demonstration of LAMP applied to detection of Cryptosporidium. Due to its specificity and simplicity, the method could become a useful diagnostic tool for epidemiologic studies of Cryptosporidium presence.