Attempts to remove nutrients from municipal wastewater have traditionally been undertaken through physical, chemical and biological methods. However, not many efforts have been made to remove nutrients in on-site wastewater treatment systems. This paper represents the experimental results of an on-site biological nutrient removal process with the potential of wastewater reuse. The performance of this pilot-scale on-site biological reactor was evaluated according to Biochemical oxygen demand (BOD5) Total suspended solid (TSS), Total nitrogen (TN) and Total phosphorus (TP) removal efficiencies. The data revealed that this form of onsite wastewater treatment system is capable of removing up to 94% of BOD5, 97% of TSS, 86 % of TN and 81% of TP. During the experimental period of one year the system was quite stable, requiring the minimum amount of maintenance and relatively low operational cost compared to other aerated wastewater treatment systems (AWTS). Based on the experimental data this on-site system can easily provide high quality reusable wastewater and can be used as a new alternative technology for the promotion of water conservation strategies.