Title

Radiosynthesis, biodistribution and micro-SPECT imaging study of dendrimer-avidin conjugate

RIS ID

79226

Publication Details

Xu, X., Zhang, Y., Wang, X., Guo, X., Zhang, X., Qi, Y. & Shen, Y. (2011). Radiosynthesis, biodistribution and micro-SPECT imaging study of dendrimer-avidin conjugate. Bioorganic and Medicinal Chemistry, 19 (5), 1643-1648.

Abstract

Partially acetylated generation five polyamidoamine (PAMAM) dendrimer (G5-Ac) was reacted with biotin and 2-(p-isothiocyanatobenzyl)-6-methyl-diethylenetria minepentaacetic acid (1B4M-DTPA), respectively to form the complex Bt-G5-Ac-1B4M which was further conjugated with avidin to give the conjugate Av-G5-Ac-1B4M. Then both of the conjugates were radiolabeled with technetium-99m (99mTc), respectively. Their in vitro cellular uptake study shows that the conjugate of Av-G5-Ac-1B4M-99mTc exhibits much higher cellular uptake in HeLa cells than that of Bt-G5-Ac-1B4M-99mTc. Accordingly the following evaluation such as in vitro/in vivo stability, biodistribution and micro-SPECT imaging was observed only for the conjugate of Av-G5-Ac-1B4M-99mTc.

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Link to publisher version (DOI)

http://dx.doi.org/10.1016/j.bmc.2011.01.036