This study investigated the removal of five selected non-phenolic trace organic contaminants (TrOC) by extracellular enzyme extract from a white-rot fungus. Except diclofenac, no other investigated non-phenolic TrOC were degraded by the extracellular enzyme extract. Improvement of enzymatic degradation of all TrOC was achieved in the presence of a redox mediator, namely, 1-hydroxybenzotriazole (HBT). However, the enhancement of degradation was mediator concentration-specific. A significant improvement in degradation at higher dosage than 0.1 mM (HBT) was achieved.